Aim: Genetic variations have been associated with physical performance. The Endothelial Nitric Oxide Synthase (eNOS) gene variants have been widely studied in this context. The aim of the present study is to compare the T786C variant of the eNOS gene in Turkish elite athletes and control groups. Material and Method: DNA samples were obtained from 52 elite athletes (45 male, 7 female) and 60 control subjects (49 male, 11 female). The T-786C variant of the eNOS gene was genotyped by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method. Results: TT, TC, CC genotypes of the T-786C variant of eNOS gene were observed in 40.0%, 48.3%, and 11.6% of control subjects and in 55.7%, 30.7% and 13.4% of elite athletes, respectively. There was not any statistically significant difference in genotype and allele frequencies of T-786C of the eNOS between the elite athlete and the control groups (p>0.05). Discussion: The present study demonstrated that the T-786C variant of the eNOS gene is not associated with study population but larger sample analyses are needed in different groups of elite athletes in order to substantiate these findings.